One step urine hCG pregnancy test is a rapid chromatographic immunoassay for the qualitative detection of hCG in human urine sample to aid in the early detection of pregnancy. Human chorionic gonadotropin (hCG) is a glycoprotein hormone excreted by the feasible placental tissue during pregnancy, is produced in urine approximately 20 days after the last menstrual interval. After 60-80 days, the level of hCG increases rapidly reaching at peak. The test uses a combination of monoclonal and polyclonal antibodies to particularly detect it take the presence of upraised levels of hCG in urine.